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KMID : 0644320030090010001
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Journal of Korean Oriental Oncology
2003 Volume.9 No. 1 p.1 ~ p.14
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Effect of Ikiyangeumhaedoc-tang on the tumor and metastasis
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Kim Jin-Sung
Yoon Sang-Hyub
Ryu Bong-Ha
Ryu Ki-Won
Yoon Seong-Woo
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Abstract
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Objective: Ikiyangeumhaedoc-tang(IYHT) has an effect of nourishing Yin(陰) and Jin(津), and has been used to cancer patient effectively. In order to prove the anticancer’s and antimetastic effect of IYHT experimentally, studies were done.
Methods: We evaluated the cytotoxic activity on HT-1080 cells as well as inhibitory effect on activity of DNA topoisomerase Ⅰ, cell adhesion, cell invasion and proliferation of HUVEC cells induced by bFGF and measured the expression of mRNA(uPA, MMP2, TIMP2), p-ERK protein, recovery effect of gap junctional intercellular communication by H₂0₂and survival time of ICR mice bearing sacoma-180.
Results: IYHT showed the inhibitory effect on DNA topoisomerase Ⅰ in the concentration of 100ug/ml, 500ug/mland the dosage-dependent inhibitory effect on the adhesion of HT-1080. The concentration of 1mg/ml of IYHT inhibited 15% of adhesion compared with control. IYHT decreased the expression of uPA, but not in MMP2, TIMP2 by RT-PCR and inhibited the expression of p-ERK effectively in the concentration of more than 500ug/ml IYHT recovered the inhibited gap junctional intercellular communication by H₂0₂ to the level of 60% of normal control in the concentration of 400ug/ml but, did not extended the mean survival time of sarcoma 180-bearing mouse.
Conclusions: It was concluded that IYHT could be applied usefully for prevention and treatment of human cancer, And also experimental study for the evaluation of molecular biological study and antimetastatic research would be recommended in the near future.
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KEYWORD
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tumor, metastasis, Ikiyangeumhaedoc-tang
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